gradientelution

Gradientelutionismandatoryinbiopolymerseparationtomatchthewiderangeofmolecularweightanalytesdifferinginhydrophobicity,chargeetc.Onlysize ...,,Duringgradientelution,thefrequenciesofsolventsignalsareshiftedduetochangesofdielectricconstantsrelatedtothemodificationofsolvent ...,AbstractAgradientmethodisrecommendedforsamplesthatcannotbeeasilyseparatedbyisocraticmethodsbecauseoftheirwidekrange.Ingra...

Gradient elution in HPLC

Gradient elution is mandatory in biopolymer separation to match the wide range of molecular weight analytes differing in hydrophobicity, charge etc. Only size ...

Gradient Elution

During gradient elution, the frequencies of solvent signals are shifted due to changes of dielectric constants related to the modification of solvent ...

Introduction to Gradient Elution

Abstract A gradient method is recommended for samples that cannot be easily separated by isocratic methods because of their wide k range. In gradient elution ...

When is Gradient Elution Better than Isocratic Elution?

The idea behind step gradients is to control individual compound elution as much as possible in an effort to maximize the separation between compounds. Also, a ...

Gradient

Definition: A separation method where the components are distributed between two phases, one of which is stationary, while the other moves in a definite ...

Gradient elution

In a process termed gradient elution, the concentration of well-retained solutes in the mobile phase is increased by constantly changing the composition, and ...

What is a Chromatography Gradient?

Gradients, used in chromatographic methods, assist with chemical separation and elution. They begin with “weak” elution conditions and end with “strong” ...

Gradient elution in normal-phase high

由 P Jandera 著作 · 2002 · 被引用 77 次 — Gradient elution is widely used for separation of complex samples in reversed-phase HPLC systems, but is less frequently applied in normal-phase HPLC, ...

Gradient Elution, Part I

由 J Dolan 著作 · 2013 · 被引用 9 次 — This rule says that changes in a chromatogram due to changes in some variable usually happen in a continuous fashion. For example, if you change ...